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KMID : 0545120080180121890
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 12 p.1890 ~ p.1894
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Influence of Deletions in the Apoemulsan Gene Cluster on A. venetianus RAG-1 Polysaccharide Biosynthesis
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Hanna Dams-Kozlowska
Mercaldi Michael P.
Ramjeawan Aruranie
Kaplan David L
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Abstract
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Apoemulsan is a biopolymer with potent emulsification activity, produced by Acinetobacter venetianus RAG-1 (RAG-1). The wee gene cluster is responsible for apoemulsan biosynthesis. The analysis of (i) a putative polysaccharide copolymerase mutant (wzc), (ii) a putative polymerase mutant (wzy), and (iii) an apoemulsan-deficient variant (2) indicated that the wee gene cluster controls the synthesis of two polysaccharides: high molecular weight (HMW) and low molecular weight (LMW). LMW polysaccharide of wee origin was present in LPS isolated from RAG-1 cells, suggesting a link to the Lipid A-core of LPS molecules. SDSPAGE analysis indicated that apoemulsan is copurified with LPS polysaccharide, with implications in the emulsification activity of RAG-1 polymer.
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KEYWORD
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RAG-1, apoemulsan, wee cluster, Wzc, Wzy
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